Composite

Part:BBa_K5317020:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)


CMV-GraR-mRuby2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 889
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1342


Design Notes

This composite part was codon optimised for human cell lines.


Source

The mRuby was retrieved from Eurofins and GraR was acquired from S.aureus <I/> .

===References===